Organs-on-Chip Facility

The ARTORG Organs-on-Chip Facility (OOCF) provides researchers at the University of Bern, the Inselspital and beyond with the infrastructure to fabricate microfluidic devices and to culture and test organs-on-chip. It is part of the ARTORG Organs-on-Chip Technologies laboratory headed by Prof. Olivier Guenat, and draws on equipment built up there over fifteen years at the interface of engineering, microfluidics and cell biology, in particular for soft lithography and for organ-on-chip operation.

The facility comprises two laboratories at the ARTORG Center, Murtenstrasse 50, 3008 Bern: the BioMEMS laboratory and the OOC Culture laboratory. Access requires prior contact with the respective laboratory manager. The OOCF is recognised by the Swiss National Science Foundation, so principal investigators holding an SNSF grant are eligible for reimbursement of user fees.

An automated organ-on-chip culture and imaging platform (ACTIO), combining robotic plate handling with confocal imaging, has recently been installed and will become available to users in due course.

BioMEMS Laboratory

The BioMEMS (bio-micro-electromechanical systems) lab comprises three parts: the design of microfluidic devices by CAD and numerical simulation, the production of microfluidic devices and the testing of those devices. The lab is equipped with an infrastructure for PDMS soft lithography, spin coating, plasma O2, laminator, hot embossing and more.

Lab manager: Mattia Panza

Organs-on-Chip Culture Laboratory

The Organs-on-Chip Culture Laboratory is a BSL-2 laboratory equipped with state-of the art cell biology equipment and specific equipment for organs-on-chip (specific incubators, …) and a 3D bioprinter.

Lab manager: Sabine Schneider

Organs-on-Chip Microscopy

Organs-on-chip technologies are microengineered devices that request specific imaging capabilities. The OOCF facility is equipped with two microscopes, a Zeiss Axio Imager M2 and a Thermo Fischer EVOS 7000. Both can image OOC in a controlled CO2 and humidity environment. The Zeiss is an upright fluorescent microscope that can be used for material sciences as well as for life sciences observations. It is located in the BioMEMS lab. The EVOS is an inverted fluorescent microscope for time lapse imaging. It is located in the OOC cell culture lab.

Microscope responsible: Amin Hajari (Zeiss Axioimager) and Sepehr Mohseni (EVOS M7000)