Lung cancer remains the leading cause of cancer-related deaths due to ineffective treatment strategies. Personalized medicine represents a solution by using genetic profiling to identify patient-specific mutations or cell based assays to predict patients’ response to chemotherapy. This project focuses on the development of microfluidic devices that best mimic in vivo conditions and that may be used in the future for such personalized medicine applications. For this purpose, spheroids are continuously perfused and different concentrations of cisplatin are administered on chip to analyze the chemosensitivity of spheroids. This study demonstrates the importance of perfusion and of co-culture systems for in vitro drug response models aimed to mimic in vivo conditions.